[NMR paper] Evolution of ?-synuclein conformation ensemble toward amyloid fibril via liquid-liquid phase separation (LLPS) as investigated by dynamic nuclear polarization-enhanced solid-state MAS NMR
Evolution of ?-synuclein conformation ensemble toward amyloid fibril via liquid-liquid phase separation (LLPS) as investigated by dynamic nuclear polarization-enhanced solid-state MAS NMR
Protein fibrillation and human neurodegenerative diseases, with a profound underlying connection suggested between them, have been the subject of intense investigations in the medical, biophysical and bio-engineering sciences. For gaining the molecular mechanistic insights into such connection, i.e., the cause and effect, atomic-resolution molecular structure information especially on the initial oligomeric states is of paramount importance, not only that on the mature amyloid fibrils....
[NMR paper] Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy
Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy
Liquid-liquid phase separation (LLPS) of protein solutions is increasingly recognised as an important phenomenon in cell biology and biotechnology. However, opalescence and concentration fluctuations render LLPS difficult to study, particularly when characterising the kinetics of the phase transition and layer separation. Here, we demonstrate the use of a probe molecule trifluoroethanol (TFE) to characterise the kinetics of protein LLPS by NMR spectroscopy. The chemical shift and...
[ASAP] Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins
Pressure-Sensitive and Osmolyte-Modulated Liquid–Liquid Phase Separation of Eye-Lens ?-Crystallins
Süleyman Cinar, Hasan Cinar, Hue Sun Chan, Roland Winter
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b13636/20190423/images/medium/ja-2018-13636x_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b13636
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/0k8PRwEgdm4
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04-24-2019 09:00 AM
Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives
From The DNP-NMR Blog:
Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives
DNP without microwave radiation? A very interesting approach.
Liu, G., et al., Photo-induced radical polarization and liquid-state dynamic nuclear polarization with fullerene nitroxide derivatives. Phys. Chem. Chem. Phys., 2017.
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12-04-2017 03:35 PM
[NMR paper] Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Mol Pharm. 2017 Jun 14;:
Authors: Kheddo P, Bramham JE, Dearman RJ, Uddin S, van der Walle CF, Golovanov AP
Abstract
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense...
Liquid state dynamic nuclear polarization of ethanol at 3.4 T (95 GHz)
From The DNP-NMR Blog:
Liquid state dynamic nuclear polarization of ethanol at 3.4 T (95 GHz)
van der Heijden, G.H., A.P. Kentgens, and P.J. van Bentum, Liquid state dynamic nuclear polarization of ethanol at 3.4 T (95 GHz). Phys Chem Chem Phys, 2014. 16(18): p. 8493-502.
http://www.ncbi.nlm.nih.gov/pubmed/24668422
Evolution of ?-synuclein conformation ensemble toward amyloid fibril via liquid-liquid phase separation (LLPS) as investigated by dynamic nuclear polarization-enhanced solid-state MAS NMR
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