Related ArticlesThe Emperor's new clothes: Myths and truths of in-cell NMR.
Arch Biochem Biophys. 2017 Mar 01;:
Authors: Pastore A, Temussi PA
Abstract
In-cell NMR is a technique developed to study the structure and dynamical behavior of biological macromolecules in their natural environment, circumventing all isolation and purification steps. In principle, the potentialities of the technique are enormous, not only for the possibility of bypassing all purification steps but, even more importantly, for the wealth of information that can be gained from directly monitoring interactions among biological macromolecules in a natural cell. Here, we review critically the promises, successes and limits of this technique as it stands now. Interestingly, many of the problems of NMR in bacterial cells stem from the artificially high concentration of the protein under study whose overexpression is anyway necessary to select it from the background. This has, as a consequence, that when overexpressed, most globular proteins, do not show an NMR spectrum, limiting the applicability of the technique to intrinsically unfolded or specifically behaving proteins. The outlook for in-cell NMR of eukaryotic cells is more promising and is possibly the most attracting aspect for the future.
PMID: 28259514 [PubMed - as supplied by publisher]
[NMR paper] Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-roysocjnls_full.gif Related Articles Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679)
Authors: Romaniuk JA, Cegelski L
Abstract
The ability to characterize bacterial cell-wall composition and structure is...
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07-07-2016 05:58 PM
[NMR paper] Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Biophys J. 2015 Mar 24;108(6):1380-9
Authors: Nygaard R, Romaniuk JA, Rice DM, Cegelski L
Abstract
Gram-positive bacteria surround themselves with a thick cell wall that is essential to cell survival and is a major target of antibiotics. Quantifying alterations in cell-wall composition are crucial to evaluating drug...
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03-27-2015 11:59 PM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
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In-cell NMR spectroscopy
In-cell NMR spectroscopy
October 2011
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br>
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12-01-2012 06:10 PM
In-cell NMR [Commentary]
In-cell NMR
Pielak, G. J., Tian, F....
Date: 2012-03-27
In PNAS, Renault et al. (1) describe an advance that will help reveal the atomic details of membrane proteins in living cells. Membrane proteins comprise one-third of all cellular proteins, including biologically crucial molecules such as ion channels, G protein-coupled receptors (GPCRs), and transporters. The GPCR superfamily alone comprises nearly half of our current drug targets. Despite their importance and prevalence, relatively few membrane protein structures are known. Of the more than 20,000 unique high-resolution protein...
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03-27-2012 08:41 PM
In-cell NMR spectroscopy
In-cell NMR spectroscopy
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 3</br>
Andres Y. Maldonado, David S. Burz, Alexander Shekhtman</br>
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The Emperor's new clothes: Myths and truths of in-cell NMR.
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