The electronic structure of the neutral isoalloxazine semiquinone within Anabaena flavodoxin: new insights from HYSCORE experiments

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The electronic structure of the neutral isoalloxazine semiquinone within Anabaena flavodoxin: new insights from HYSCORE experiments

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03-09-2012, 09:16 AM

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The electronic structure of the neutral isoalloxazine semiquinone within Anabaena flavodoxin: new insights from HYSCORE experiments

The electronic structure of the neutral isoalloxazine semiquinone within Anabaena flavodoxin: new insights from HYSCORE experiments

Publication year: 2012
Source:Journal of Magnetic Resonance

Jesús I. Martínez, Pablo J. Alonso, Milagros Medina

A complete study of Anabaena flavodoxin in the neutral semiquinone state by means of the EPR pulse technique HYSCORE is here presented. The results provide new information about the hyperfine interactions of the unpaired electronic spin and the nuclei in the isoalloxazine ring. This allows a better knowledge of the electronic structure of the neutral flavin radical within the protein. Combination of these results with other previously obtained by using other EPR related techniques allowed producing a very precise mapping of the flavin spin distribution in the neutral semiquinone state. This information can be very useful for determining the relationship between the electronic structure and mechanisms in flavoproteins. An experimental protocol for measuring the electronic structure details available to date is suggested.
Graphical Abstract

Graphical abstract Highlights

? A complete characterization of flavin centres from HYSCORE spectroscopy. ? New data on the hyperfine coupling in the flavin ring are obtained. ? A protocol of EPR techniques allows revealing the flavin electronic structure.

Source: Journal of Magnetic Resonance

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