This study introduces a conceptually new solvent suppression scheme with adiabatic inversion pulses for 1H-detected multidimensional solid-state NMR (SSNMR) of biomolecules and other systems, which is termed â??Solvent suppression of Liquid signal with Adiabatic Pulseâ?? (SLAP). 1H-detected 2D 13C/1H SSNMR data of uniformly 13C- and 15N-labeled GB1 sample using ultra-fast magic angle spinning at a spinning rate of 60Â*kHz demonstrated that the SLAP scheme showed up to 3.5-fold better solvent suppression performance over a traditional solvent-suppression scheme for SSNMR, MISSISSIPPI (Zhou and Rienstra, J Magn Reson 192:167â??172, 2008) with 2/3 of the average RF power.
Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments
Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments
Abstract
Recent methyl adiabatic relaxation dispersion experiments provide examination of conformational dynamics across a very wide timescale (102â??105Â*sâ??1) and, particularly, provide insight into the hydrophobic core of proteins and allosteric effects associated with modulators. The experiments require efficient decoupling of 1H and 13C spin interactions, and some artifacts have been discovered, which are associated with the design of the proton...
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04-24-2020 11:56 AM
Solvent suppression in solid-state DNP NMR using Electronic Mixing-Mediated Annihilation (EMMA) #DNPNMR
From The DNP-NMR Blog:
Solvent suppression in solid-state DNP NMR using Electronic Mixing-Mediated Annihilation (EMMA) #DNPNMR
Ziarelli, Fabio, Pierre Thureau, Stéphane Viel, and Giulia Mollica. “Solvent Suppression in Solid-State DNP NMR Using Electronic Mixing-Mediated Annihilation (EMMA).” Magnetic Resonance in Chemistry, January 23, 2020.
https://doi.org/10.1002/mrc.5001.
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02-29-2020 09:52 PM
[U. of Ottawa NMR Facility Blog] Improved Solvent Suppression with Composite Pulses
Improved Solvent Suppression with Composite Pulses
A hard rf pulse delivers an rf field to the NMR sample inside of the coil. The rf field is not perfectly homogeneous nor does it end abruptly at the edge of the coil. When a long, selective, low power presaturation pulse is given to suppress a water signal, the water in the coil may be fully saturated whereas water outside of the coil will not be. Furthermore, unsaturated water outside of the coil may be outside of the carefully shimmed region of the magnet and give rise to a broadened residual signal when presaturation is used prior to a...
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01-05-2018 07:18 AM
Solvent suppression in DNP enhanced solid state NMR #DNPNMR
From The DNP-NMR Blog:
Solvent suppression in DNP enhanced solid state NMR #DNPNMR
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Yarava, J.R., et al., Solvent suppression in DNP enhanced solid state NMR. J Magn Reson, 2017. 277: p. 149-153.
https://www.ncbi.nlm.nih.gov/pubmed/28288417
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06-30-2017 06:14 PM
[NMR paper] An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
Related Articles An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Nov 11;
Authors: Mance D, Sinnige T, Kaplan M, Narasimhan S, Daniëls M, Houben K, Baldus M, Weingarth M
Abstract
(1) H-detection can greatly improve spectral sensitivity in biological solid-state NMR (ssNMR), thus allowing the study of larger...
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11-12-2015 11:28 PM
[NMR paper] Low-power polarization transfer between deuterons and spin-1/2 nuclei using adiabatic (RESPIRATION)CP in solid-state NMR.
Low-power polarization transfer between deuterons and spin-1/2 nuclei using adiabatic (RESPIRATION)CP in solid-state NMR.
Related Articles Low-power polarization transfer between deuterons and spin-1/2 nuclei using adiabatic (RESPIRATION)CP in solid-state NMR.
Phys Chem Chem Phys. 2014 Jan 13;
Authors: Jain SK, Nielsen AB, Hiller M, Handel L, Ernst M, Oschkinat H, Akbey U, Nielsen NC
Abstract
Establishing high-resolution structures of biological macromolecules in heterogeneous environments by MAS solid-state NMR is an important...
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01-15-2014 05:16 PM
[Question from NMRWiki Q&A forum] How can I tell if and what kind of solvent suppression was used?
How can I tell if and what kind of solvent suppression was used?
I have some older 2D HSQC 1H-15N data that was run by a NMR tech. Is there a way I can tell what kind of solvent suppression was used by looking through the acquisition files? Like I know I can open them up as text documents and see a lot of the experimental parameters, but I don't know if solvent suppression is listed in one of those parameters.
Thanks! -Brian W.
Check if somebody has answered this question on NMRWiki QA forum
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11-23-2013 04:05 AM
Solvent signal suppression in NMR
Solvent signal suppression in NMR
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 25 January 2010</br>
Gang, Zheng , William S., Price</br>
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Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR
Linear Mode
