An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors
An efficient method and device for transfer of semisolid materials into solid-state NMR spectroscopy rotors
Publication date: Available online 29 January 2016 Source:Journal of Magnetic Resonance</br> Author(s): Grant S. Hisao, Michael A. Harland, Robert A. Brown, Deborah A. Berthold, Thomas E. Wilson, Chad M. Rienstra</br> The study of mass-limited biological samples by magic angle spinning (MAS) solid-state NMR spectroscopy critically relies upon the high-yield transfer of material from a biological preparation into the MAS rotor. This issue is particularly important for maintaining biological activity and hydration of semi-solid samples such as membrane proteins in lipid bilayers, pharmaceutical formulations, microcrystalline proteins and protein fibrils. Here we present protocols and designs for rotor-packing devices specifically suited for packing hydrated samples into Pencil-style 1.6 mm, 3.2 mm standard, and 3.2 mm limited speed MAS rotors. The devices are modular and therefore readily adaptable to other rotor and/or ultracentrifugation tube geometries. Graphical abstract http://origin-ars.els-cdn.com/conten...000501-fx1.jpg</br></br> </br></br> Source: Journal of Magnetic Resonance |
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