NMR paper An efficient 3D NMR technique for correlating the proton and 15N backbone amide reson

		BioNMR > Educational resources > Journal club
[NMR paper] An efficient 3D NMR technique for correlating the proton and 15N backbone amide reson

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:16 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

An efficient 3D NMR technique for correlating the proton and 15N backbone amide reson

An efficient 3D NMR technique for correlating the proton and 15N backbone amide resonances with the alpha-carbon of the preceding residue in uniformly 15N/13C enriched proteins.

Related Articles An efficient 3D NMR technique for correlating the proton and 15N backbone amide resonances with the alpha-carbon of the preceding residue in uniformly 15N/13C enriched proteins.

J Biomol NMR. 1991 May;1(1):99-104

Authors: Bax A, Ikura M

A 3D NMR technique is described which correlates the amide proton and nitrogen resonances of an amino acid residue with the C alpha chemical shift of its preceding residue. The technique uses a relay mechanism, transferring magnetization from 15N to 13C alpha via the intervening carbonyl nucleus. This method for obtaining sequential connectivity is less sensitive to large line widths than the alternative HNCA experiment. The technique is demonstrated for the protein calmodulin, complexed with a 26 amino acid fragment of skeletal muscle myosin light chain kinase.

PMID: 1668719 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Temperature dependence of protein backbone motion from carbonyl 13C and amide 15N NMR Temperature dependence of protein backbone motion from carbonyl 13C and amide 15N NMR relaxation. Related Articles Temperature dependence of protein backbone motion from carbonyl 13C and amide 15N NMR relaxation. J Magn Reson. 2005 May;174(1):43-53 Authors: Chang SL, Tjandra N The NMR spin-lattice relaxation rate (R1) and the rotating-frame spin-lattice relaxation rate (R1rho) of amide 15N and carbonyl 13C (13C') of the uniformly 13C- and 15N-labeled ubiquitin were measured at different temperatures and field strengths to investigate the...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] A combinatorial selective labeling method for the assignment of backbone amide NMR re A combinatorial selective labeling method for the assignment of backbone amide NMR resonances. Related Articles A combinatorial selective labeling method for the assignment of backbone amide NMR resonances. J Am Chem Soc. 2004 Apr 28;126(16):5020-1 Authors: Parker MJ, Aulton-Jones M, Hounslow AM, Craven CJ A combinatorial selective labeling (CSL) method is presented for the assignment of backbone amide NMR resonances, which has a particular application in the identification of protein-ligand interaction sites. The method builds on the dual...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Computational assignment of protein backbone NMR peaks by efficient bounding and filt Computational assignment of protein backbone NMR peaks by efficient bounding and filtering. Related Articles Computational assignment of protein backbone NMR peaks by efficient bounding and filtering. J Bioinform Comput Biol. 2003 Jul;1(2):387-409 Authors: Lin G, Xu D, Chen ZZ, Jiang T, Wen J, Xu Y NMR resonance assignment is one of the key steps in solving an NMR protein structure. The assignment process links resonance peaks to individual residues of the target protein sequence, providing the prerequisite for establishing intra- and...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra. Related Articles Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra. J Biomol NMR. 2003 Mar;25(3):217-23 Authors: van Rossum BJ, Castellani F, Pauli J, Rehbein K, Hollander J, de Groot HJ, Oschkinat H In this paper, we present a strategy for the (1)H(N) resonance assignment in solid-state magic-angle spinning (MAS) NMR, using the alpha-spectrin SH3 domain as an example. A novel 3D...	nmrlearner	Journal club	0	11-24-2010 09:01 PM
[NMR paper] An efficient branch-and-bound algorithm for the assignment of protein backbone NMR pe An efficient branch-and-bound algorithm for the assignment of protein backbone NMR peaks. Related Articles An efficient branch-and-bound algorithm for the assignment of protein backbone NMR peaks. Proc IEEE Comput Soc Bioinform Conf. 2002;1:165-74 Authors: Lin G, Xu D, Chen ZZ, Jiang T, Wen J, Xu Y NMR resonance assignment is one of the key steps in solving an NMR protein structure. The assignment process links resonance peaks to individual residues of the target protein sequence, providing the prerequisite for establishing intra- and...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
Backbone Amide Dynamics Studies of Apo-L75F-TrpR, a Temperature-Sensitive Mutant of t Backbone Amide Dynamics Studies of Apo-L75F-TrpR, a Temperature-Sensitive Mutant of the Tryptophan Repressor Protein (TrpR): Comparison with the 15N NMR Relaxation Profiles of Wild-Type and A77V Mutant Apo-TrpR Repressors http://pubs.acs.org//appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi100508u/aop/images/medium/bi-2010-00508u_0005.gifBiochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). More...	nmrlearner	Journal club	0	08-31-2010 10:50 PM
[NMR paper] Backbone dynamics, amide hydrogen exchange, and resonance assignments of the DNA meth Backbone dynamics, amide hydrogen exchange, and resonance assignments of the DNA methylphosphotriester repair domain of Escherichia coli Ada using NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Backbone dynamics, amide hydrogen exchange, and resonance assignments of the DNA methylphosphotriester repair domain of Escherichia coli Ada using NMR. Biochemistry. 1996 Jul 23;35(29):9335-48 Authors: Habazettl J, Myers LC, Yuan F, Verdine GL, Wagner G The 10kDa amino-terminal fragment of...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Kinetics of amide proton exchange in parvalbumin studied by 1H 2-D NMR. A comparison Kinetics of amide proton exchange in parvalbumin studied by 1H 2-D NMR. A comparison of the calcium and magnesium loaded forms. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Kinetics of amide proton exchange in parvalbumin studied by 1H 2-D NMR. A comparison of the calcium and magnesium loaded forms. Biochimie. 1992 Sep-Oct;74(9-10):837-44 Authors: Baldellon C, Padilla A, Cavé A The amide proton exchange rates have been measured for the pike parvalbumin loaded...	nmrlearner	Journal club	0	08-21-2010 11:45 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off