The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.
 

The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.

Related Articles The effects of acute lipopolysaccharide challenge on dairy goat liver metabolism assessed with (1) HNMR metabonomics.

J Anim Physiol Anim Nutr (Berl). 2016 Feb 5;

Authors: Wang LF, Jia SD, Yang GQ, Liu RY, Yang GY, Li M, Zhu HS, Wang YY, Han LQ


Abstract
We investigated the mechanisms mediating hepatic metabolic responses to an acute lipopolysaccharide (LPS) challenge in goats. Guanzhong dairy goats (15) were randomly divided into three groups: control (CTL, saline, 0.2*ml/kg BW), lower dose LPS (LPS-L, 20*?g/kg BW) and higher dose LPS (LPS-H, 40*?g/kg BW). All injections were administered intraperitoneally twice with a 24-h interval. Forty-eight hours after the first injection, blood samples were collected to extract plasma for biochemical analysis, and liver tissues were biopsied and stored in liquid nitrogen for metabonomics analysis. We found that plasma levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin increased (p*