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Default Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.

Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.

Effect of organochlorine pesticides exposure on the maize root metabolome assessed using high-resolution magic-angle spinning (1)H NMR spectroscopy.

Environ Pollut. 2016 Apr 28;214:539-548

Authors: Blondel C, Khelalfa F, Reynaud S, Fauvelle F, Raveton M

Abstract
(1)H-HRMAS NMR-based metabolomics was used to better understand the toxic effects on maize root tips of organochlorine pesticides (OCPs), namely lindane (?HCH) and chlordecone (CLD). Maize seedlings were exposed to 2.5*?M ?HCH (mimicking basic environmental contaminations) for 7 days and compared to 2.5*?M CLD and 25*?M ?HCH for 7 days (mimicking hot spot contaminations). The (1)H-HRMAS NMR-based metabolomic profiles provided details of the changes in carbohydrates, amino acids, tricarboxylic acid (TCA) cycle intermediates and fatty acids with a significant separation between the control and OCP-exposed root tips. First of all, alterations in the balance between glycolysis/gluconeogenesis were observed with sucrose depletion and with dose-dependent fluctuations in glucose content. Secondly, observations indicated that OCPs might inactivate the TCA cycle, with sizeable succinate and fumarate depletion. Thirdly, disturbances in the amino acid composition (GABA, glutamine/glutamate, asparagine, isoleucine) reflected a new distribution of internal nitrogen compounds under OCP stress. Finally, OCP exposure caused an increase in fatty acid content, concomitant with a marked rise in oxidized fatty acids which could indicate failures in cell integrity and vitality. Moreover, the accumulation of asparagine and oxidized fatty acids with the induction of LOX3 transcription levels under OCP exposure highlighted an induction of protein and lipid catabolism. The overall data indicated that the effect of OCPs on primary metabolism could have broader physiological consequences on root development. Therefore, (1)H-HRMAS NMR metabolomics is a sensitive tool for understanding molecular disturbances under OCP exposure and can be used to perform a rapid assessment of phytotoxicity.


PMID: 27131813 [PubMed - as supplied by publisher]



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