BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 11-19-2010, 08:32 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Editing and diagonal peak suppression in three-dimensional HCCH protein NMR correlati

Editing and diagonal peak suppression in three-dimensional HCCH protein NMR correlation experiments.

Related Articles Editing and diagonal peak suppression in three-dimensional HCCH protein NMR correlation experiments.

J Biomol NMR. 2001 Jan;19(1):69-73

Authors: Meissner A, Sørensen OW

A novel three-dimensional (3D) HCCH NMR experiment is introduced. It involves 13C-13C COSY or TOCSY coherence transfer plus two independent editing steps according to the number of protons attached to the individual carbons before and after the 13C-13C homonuclear mixing. This double editing leads to simplification of HCCH protein side chain spectra that otherwise are prone to spectral overlap. Another interesting feature is amino acid selectivity, i.e. that the presence of certain correlations in a doubly edited HCCH subspectrum gives a clue as to assignment to a particular subgroup of amino acids or segments thereof. Finally, the selection of two different multiplicities in the two editing steps leads to diagonal peak suppression in the 1H-1H (3D spectrum recorded with two 1H and one 13C dimension) or the 13C-13C (3D spectrum recorded with one 1H and two 13C dimensions) two-dimensional projection. The new experiment is demonstrated using a 13C,15N-labeled protein sample, chymotrypsin inhibitor 2, at 500 MHz.

PMID: 11246856 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Suppression of sampling artefacts in high-resolution four-dimensional NMR spectra using Signal Separation Algorithm
Suppression of sampling artefacts in high-resolution four-dimensional NMR spectra using Signal Separation Algorithm Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 20 October 2011</br> Jan*Stanek, Rafal*Augustyniak, Wiktor*Ko?mi?ski</br> The development of non-uniform sampling (NUS) strategies permits to obtain high-dimensional spectra with increased resolution in significantly reduced experimental time. We extended a previously proposed signal separation algorithm (SSA) to process sparse four-dimensional NMR data. It is employed for two experiments...
nmrlearner Journal club 0 10-22-2011 10:16 AM
[NMR paper] Suppression of diagonal peaks in three-dimensional protein NMR TROSY-type HCCH correl
Suppression of diagonal peaks in three-dimensional protein NMR TROSY-type HCCH correlation experiments. Related Articles Suppression of diagonal peaks in three-dimensional protein NMR TROSY-type HCCH correlation experiments. J Magn Reson. 2000 May;144(1):171-4 Authors: Meissner A, Sorensen OW A novel method for suppression of (13)C-(13)C diagonal peaks without sensitivity loss in three-dimensional HCCH TROSY-type NMR correlation experiments involving aromatic side chains in proteins (Pervushin et al., J. Am. Chem. Soc. 120, 6394-6400 (1998))...
nmrlearner Journal club 0 11-18-2010 09:15 PM
[NMR paper] Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled prote
Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins. Related Articles Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins. J Magn Reson. 1999 Oct;140(2):499-503 Authors: Meissner A, Sørensen OW A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of 15N-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR paper] The 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY NMR experiment with diagonal peak suppression
The 3D NOESY--ZQ-TROSY NMR experiment with diagonal peak suppression. Related Articles The 3D NOESY--ZQ-TROSY NMR experiment with diagonal peak suppression. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9607-12 Authors: Pervushin KV, Wider G, Riek R, Wüthrich K In our 3D NOESY--ZQ-TROSY experiment, the TROSY principle (transverse relaxation-optimized spectroscopy) is used in three-dimensional (3D) (15)N-resolved nuclear Overhauser enhancement spectroscopy (NOESY), which enables resonance assignments by sequential nuclear Overhauser effects and...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR paper] Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(1
Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins. Related Articles Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins. J Magn Reson. 1999 Aug;139(2):447-50 Authors: Meissner A, Sorensen OW Improved methods for three-dimensional TROSY-Type HCCH correlation involving protons of negligible CSA are presented. The TROSY approach differs from the conventional approach of heteronuclear decoupling in evolution and detection periods by...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP
Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY. Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY. J Magn Reson. 1998 Dec;135(2):288-97 Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...
nmrlearner Journal club 0 11-17-2010 11:15 PM
[Stan NMR blog] Wavelet-based filtering applied to the diagonal suppression of 2D NMR data sets
Wavelet-based filtering applied to the diagonal suppression of 2D NMR data sets A novel 2D-NMR data-evaluation algorithm by Carlos Cobas. More...
nmrlearner News from NMR blogs 0 08-21-2010 06:14 PM
[NMR paper] Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of p
Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of protein-water interactions. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Editing of chemical exchange-relayed NOEs in NMR experiments for the observation of protein-water interactions. J Magn Reson. 1999 Feb;136(2):214-8 Authors: Melacini G, Kaptein R, Boelens R An experimental approach for the editing of exchange-relayed NOEs in water-selective NOE experiments is presented. The...
nmrlearner Journal club 0 08-21-2010 04:03 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 04:54 AM.


Map