Dynamics of uniformly labelled solid proteins between 100 and 300 K: A 2D (2)H-(13)C MAS NMR approach
 

Dynamics of uniformly labelled solid proteins between 100 and 300 K: A 2D (2)H-(13)C MAS NMR approach

We describe a ²H based MAS nuclear magnetic resonance (NMR) method to obtain site-specific molecular dynamics of biomolecules. The method utilizes the use of deuterium nucleus as a spin label that is proven to be very useful in dynamics studies of solid biological and functional materials. The aim is to understand overall characteristics of protein backbone and side-chain motions for CD(3), CD(2) and CD groups, in terms of timescale, type and activation energy of the underlying processes....

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