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Default Dynamics of Cations around DNA and Protein as Revealed by (23)Na Diffusion NMR Spectroscopy

Dynamics of Cations around DNA and Protein as Revealed by (23)Na Diffusion NMR Spectroscopy

Counterions are vital for the structure and function of biomolecules. However, the behavior of counterions remains elusive due to the difficulty in characterizing mobile ions. Here, we demonstrate that the dynamics of cations around biological macromolecules can be revealed by ^(23)Na diffusion nuclear magnetic resonance (NMR) spectroscopy. NMR probe hardware capable of generating strong magnetic field gradients enables ^(23)Na NMR-based diffusion measurements for Na^(+) ions in solutions of...

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