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Default Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry.

Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry.

Related Articles Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry.

Solid State Nucl Magn Reson. 2019 Mar 22;100:70-76

Authors: Salnikov ES, Aussenac F, Abel S, Purea A, Tordo P, Ouari O, Bechinger B

Abstract
Whereas specially designed dinitroxide biradicals, reconstitution protocols, oriented sample geometries and NMR probes have helped to much increase the DNP enhancement factors of membrane samples they still lag considerably behind those obtained from glasses made of protein solutions. Here we show that not only the MAS rotor material but also the distribution of the membrane samples within the NMR rotor have a pronounced effect on the DNP enhancement. These observations are rationalized with the cooling efficiency and the internal properties of the sample, monitored by their T1 relaxation, microwave ON versus OFF signal intensities and DNP effect. The data are suggestive that for membranes the speed of cooling has a pronounced effect on the membrane properties and concomitantly the distribution of biradicals within the sample.


PMID: 30995597 [PubMed - as supplied by publisher]



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