Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
 

Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy

In-cell NMR spectroscopy is a powerful approach to study protein structure and function in the native cellular environment. It provides precious insights into the folding, maturation, interactions, and ligand binding of important pharmacological targets directly in human cells. However, its widespread application is hampered by the fact that soluble globular proteins often interact with large cellular components, causing severe line broadening in conventional heteronuclear NMR experiments....

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