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Default Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy.

Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy.

Related Articles Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy.

Analyst. 2018 Jan 02;:

Authors: Sepuru KM, Iwahara J, Rajarathnam K

Abstract
Two NMR observables, the N?H3+ peak in the HISQC spectrum and N? chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.


PMID: 29292440 [PubMed - as supplied by publisher]



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