[NMR paper] Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

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Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Related Articles Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Magn Reson Chem. 2020 Nov 08;:

Authors: Vugmeyster L, Ostrovsky D

Abstract
We describe a new method for measuring molecular dynamics based on the deuterium solid-state nuclear magnetic resonance (NMR) quadrupolar order rotating frame relaxation rate R1?,Q under static conditions. The observed quadrupolar order coherence is created using the broad-band Jeener-Broekaert excitation and is locked with a weak radio frequency (RF) field. We describe the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid-? (1-40) fibrils phosphorylated at the serine-8 position. The R1?,Q rate is sensitive to the rotameric exchange mode. For biological samples, the low spin-lock field in the 5- to 10-kHz range has the advantage of avoiding sample heating and dehydration. Thus, it provides an alternative to approaches based on single-quantum coherence, which require larger spin-lock fields.


PMID: 33161607 [PubMed - as supplied by publisher]



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