Deuteration and selective labeling of alanine methyl groups of β 2 -adrenergic receptor expressed in a baculovirus-insect cell expression system
 

Deuteration and selective labeling of alanine methyl groups of β 2 -adrenergic receptor expressed in a baculovirus-insect cell expression system

Abstract

G protein-coupled receptors (GPCRs) exist in equilibrium between multiple conformations, and their populations and exchange rates determine their functions. However, analyses of the conformational dynamics of GPCRs in lipid bilayers are still challenging, because methods for observations of NMR signals of large proteins expressed in a baculovirus-insect cell expression system (BVES) are limited. Here, we report a method to incorporate methyl-13C1H3-labeled alanine with >â??45% efficiency in highly deuterated proteins expressed in BVES. Application of the method to the NMR observations of β2-adrenergic receptor in micelles and in nanodiscs revealed the ligand-induced conformational differences throughout the transmembrane region of the GPCR.



Source: Journal of Biomolecular NMR