Determination of muscle protein synthesis rates in fish using 2H2O and 2H NMR analysis of alanine
 

Determination of muscle protein synthesis rates in fish using 2H2O and 2H NMR analysis of alanine

Publication date: Available online 11 July 2016
Source:Analytical Biochemistry</br>
Author(s): Cátia Marques, Filipa Viegas, João Rito, John Jones, Ivan Viegas</br>
Following administration of deuterated water (2H2O), the fractional synthetic rate (FSR) of a given endogenous protein can be estimated by 2H-enrichment quantification of its alanine residues. Currently, this is measured by mass spectrometry following a derivatization procedure. Muscle FSR was measured by 1H/2H NMR analysis of alanine from seabass kept for 6 days in 5% 2H-enriched saltwater, following acid hydrolysis and amino acid isolation by cation-exchange chromatography of muscle tissue. The analysis is simple and robust, and provides precise measurements of excess alanine 2H-enrichment in the 0.1–0.4% range from 50*mmol of alanine recovered from muscle protein.
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