Detection and characterization of a folding intermediate in barnase by NMR.
Detection and characterization of a folding intermediate in barnase by NMR.
http://www.ncbi.nlm.nih.gov/corehtml...-lo_nature.gif Related Articles Detection and characterization of a folding intermediate in barnase by NMR. Nature. 1990 Aug 2;346(6283):488-90 Authors: Bycroft M, Matouschek A, Kellis JT, Serrano L, Fersht AR Protein engineering is being developed for mapping the energetics and pathway of protein folding. From kinetic studies on wild-type and mutant proteins, the sequence and energetics of formation of tertiary interactions of side chains can be mapped and the formation of secondary structure inferred. Here we cross-check and complement results from this approach by using a recently developed procedure that traps and characterizes secondary structure in intermediate states using 1H NMR. The refolding of barnase is shown to be a multiphasic process in which the secondary structure in alpha-helices and beta-sheets and some turns is formed more rapidly than is the overall folding. PMID: 2377210 [PubMed - indexed for MEDLINE] Source: PubMed |
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