Related ArticlesHigh-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations.
J Magn Reson. 2014 Feb 20;242C:49-56
Authors: Zhang Z, Huang Y, Smith PE, Wang K, Cai S, Chen Z
Abstract
Heteronuclear NMR spectroscopy is an extremely powerful tool for determining the structures of organic molecules and is of particular significance in the structural analysis of proteins. In order to leverage the method's potential for structural investigations, obtaining high-resolution NMR spectra is essential and this is generally accomplished by using very homogeneous magnetic fields. However, there are several situations where magnetic field distortions and thus line broadening is unavoidable, for example, the samples under investigation may be inherently heterogeneous, and the magnet's homogeneity may be poor. This line broadening can hinder resonance assignment or even render it impossible. We put forth a new class of pulse sequences for obtaining high-resolution heteronuclear spectra in magnetic fields with unknown spatial variations based on distant dipolar field modulations. This strategy's capabilities are demonstrated with the acquisition of high-resolution 2D gHSQC and gHMBC spectra. These sequences' performances are evaluated on the basis of their sensitivities and acquisition efficiencies. Moreover, we show that by encoding and decoding NMR observables spatially, as is done in ultrafast NMR, an extra dimension containing J-coupling information can be obtained without increasing the time necessary to acquire a heteronuclear correlation spectrum. Since the new sequences relax magnetic field homogeneity constraints imposed upon high-resolution NMR, they may be applied in portable NMR sensors and studies of heterogeneous chemical and biological materials.
PMID: 24607822 [PubMed - as supplied by publisher]
High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations
High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations
Publication date: Available online 20 February 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Zhiyong Zhang , Yuqing Huang , Pieter E.S. Smith , Kaiyu Wang , Shuhui Cai , Zhong Chen</br>
Heteronuclear NMR spectroscopy is an extremely powerful tool for determining the structures of organic molecules and is of particular significance in the structural analysis of proteins. In order to leverage the method’s potential for structural...
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02-20-2014 05:15 AM
[NMR paper] Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling.
Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling.
Related Articles Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling.
Chem Commun (Camb). 2013 Dec 23;
Authors: Helge Meyer N, Zangger K
Abstract
Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins. Here we show that homonuclear...
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12-25-2013 03:39 PM
High Resolution Para-Hydrogen Induced Polarization in Inhomogeneous Magnetic Fields
From the The DNP-NMR Blog:
High Resolution Para-Hydrogen Induced Polarization in Inhomogeneous Magnetic Fields
Buljubasich, L., et al., High Resolution Para-Hydrogen Induced Polarization in Inhomogeneous Magnetic Fields. J. Magn. Reson., 2013(0).
http://dx.doi.org/10.1016/j.jmr.2013.02.008
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04-15-2013 08:52 AM
Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot
From the The DNP-NMR Blog:
Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot
Safiullin, K., C. Talbot, and P.J. Nacher, Achieving high spatial resolution and high SNR in low-field MRI of hyperpolarised gases with Slow Low Angle SHot. J Magn Reson, 2013. 227(0): p. 72-86.
http://dx.doi.org/10.1016/j.jmr.2012.11.025
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04-15-2013 08:52 AM
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Abstract Well-resolved 2Hâ??13C correlation spectra, reminiscent of 1Hâ??13C correlations, are obtained for perdeuterated ubiquitin and for perdeuterated outer-membrane protein G (OmpG) from E. coli by exploiting the favorable lifetime of 2H double-quantum (DQ) states. Sufficient signal-to-noise was achieved due to the short deuterium T 1, allowing for high repetition rates and enabling 3D experiments with a 2Hâ??13C transfer step in a reasonable time....
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11-01-2011 01:52 AM
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
Abstract Magic-angle spinning (MAS) solid-state NMR (SSNMR) spectroscopy of uniformly-13C,15N labeled protein samples provides insight into atomic-resolution chemistry and structure. Data collection efficiency has advanced remarkably in the last decade; however, the study of larger proteins is still challenged by relatively low resolution in comparison to solution NMR. In this study, we present a systematic analysis of SSNMR protein spectra acquired at 11.7, 17.6 and 21.1 Tesla (1H frequencies of...
High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations.
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