Danio rerio Oocytes for Eukaryotic In-Cell NMR.
 

Danio rerio Oocytes for Eukaryotic In-Cell NMR.

Related Articles Danio rerio Oocytes for Eukaryotic In-Cell NMR.

Biochemistry. 2021 Feb 03;:

Authors: Thole JF, Fadero TC, Bonin JP, Stadmiller SS, Giudice JA, Pielak GJ

Abstract
Understanding how the crowded and complex cellular milieu affects protein stability and dynamics has only recently become possible by using techniques such as in-cell nuclear magnetic resonance. However, the combination of stabilizing and destabilizing interactions makes simple predictions difficult. Here we show the potential of Danio rerio oocytes as an in-cell nuclear magnetic resonance model that can be widely used to measure protein stability and dynamics. We demonstrate that in eukaryotic oocytes, which are 3-6-fold less crowded than other cell types, attractive chemical interactions still dominate effects on protein stability and slow tumbling times, compared to the effects of dilute buffer.


PMID: 33534998 [PubMed - as supplied by publisher]



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