Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 11 March 2011
G., Mathies , H., Blok , J.A.J.M., Disselhorst , P., Gast , H., van der Meer , ...
The 275 GHz electron-paramagnetic-resonance spectrometer we reported on in 2004 has been equipped with a new probe head, which contains a cavity especially designed for operation in continuous-wave mode. The sensitivity and signal stability that is achieved with this new probe head is illustrated with 275 GHz continuous-wave spectra of a 1 mM frozen solution of the complex Fe(III)-ethylenediamine tetra-acetic acid (EDTA) and of 10 mM frozen solutions of the protein rubredoxin, which contains Fe3+ in its active site, from three different organisms. The high quality of the spectra of the rubredoxins allows the determination of the zero-field splitting parameters... Graphical abstract
*Graphical abstract:**Research Highlights:*? 275 GHz continuous-wave EPR spectroscopy on high-spin Fe3+ systems using a specialized single-mode cavity. ? Sensitivity and signal stability are illustrated on dilute solutions of Fe(III)-EDTA and rubredoxin. ? Zero-field-splitting parameters and g-anisotropy were determined accu-rately by simulation of the spectra
Relaxation theory of nuclear singlet states in two spin-1/2 systems
Relaxation theory of nuclear singlet states in two spin-1/2 systems
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 56, Issue 3, April 2010, Pages 217-231</br>
Giuseppe*Pileio</br>
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Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
J Magn Reson. 2011 Apr;209(2):123-30
Authors: Castillo AM, Patiny L, Wist J
The computational cost for the simulation of NMR spectra grows exponentially with the number of nuclei. Today, the memory available to store the Hamiltonian limits the size of the system that can be studied. Modern computers enable to tackle systems containing up to 13 spins , which obviously does not...
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07-23-2011 08:54 AM
[NMR paper] Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interac
Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions.
Related Articles Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions.
J Magn Reson. 2004 Nov;171(1):163-70
Authors: Shrot Y, Shapira B, Frydman L
A new protocol for acquiring multidimensional NMR spectra within a single scan is introduced and illustrated. The approach relies on applying a pair of frequency-chirped excitation and storage pulses in combination with echoing magnetic field gradients, in order to impart...
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11-24-2010 10:03 PM
[NMR paper] Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(L
Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains.
Related Articles Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains.
Biochemistry. 2001 Aug 14;40(32):9596-604
Authors: Schüler W, Kloiber K, Matt T, Bister K, Konrat R
The solution structure of quail CRP2(LIM2) was significantly improved by using an increased number of NOE constraints obtained from a 13C,15N-labeled...
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[NMR paper] Protein NMR spin trapping with [methyl-13C(3)]-MNP: application to the tyrosyl radica
Protein NMR spin trapping with -MNP: application to the tyrosyl radical of equine myoglobin.
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Free Radic Biol Med. 2001 Aug 1;31(3):383-90
Authors: Bose-Basu B, DeRose EF, Chen YR, Mason RP, London RE
Direct spin trapping studies of protein radical adducts are limited as a consequence of the long rotational correlation times and consequent broadening of the ESR resonances. It can be difficult to determine both the nature and number of...
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11-19-2010 08:44 PM
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Me
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Methods
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 13 April 2010</br>
Harald, Walderhaug , Olle, Söderman , Daniel, Topgaard</br>
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08-16-2010 03:50 AM
Sequential Assignment of Spin systems
hi,
I am new to the protein NMR Field.I want to assign spin numbers to 120 amonoacid residues of a protein.I am cluless how to assing these number to each.Could anyone help me? Here is the primary sequence f a protein domain-
ISLLAQRQQF WIQLEFHSRI TRGERHGVID HVGLGVQSQQ RSNGFLTSLC ILRPHYASLS LALEKAQLHS LLCEETDGEG TLEYGFMGQV KSRFTDLSRP NLFCRHLGQL LPLLRVCRDV
I am expecing your help.Thank you.
Continuous-wave EPR at 275 GHz: Application to high-spin Fe3+ systems
Linear Mode
