A comprehensive assessment of selective amino acid (15)N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
 

A comprehensive assessment of selective amino acid (15)N-labeling in human embryonic kidney 293 cells for NMR spectroscopy

A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. Thus, mammalian expression systems are often employed to characterize structure/function relationships using NMR spectroscopy. Here we define the selective isotope labeling of secreted, post-translationally modified proteins using human embryonic kidney (HEK)293 cells. We determined that alpha-[^(15)N]- atoms from 10 amino acids experience minimal metabolic scrambling (C, F,...

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