Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease u
 

Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy.

http://www.ncbi.nlm.nih.gov/corehtml...PubMedLink.gif Related Articles Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy.

FEBS Lett. 1991 Apr 9;281(1-2):33-8

Authors: Baldisseri DM, Pelton JG, Sparks SW, Torchia DA

Complete proton and carbon sidechain assignments are reported for 22 lysine and 11 leucine residues in staphylococcal nuclease, an enzyme with 149 residues. These assignments are readily obtained in a direct manner from the correlations observed in the 3D HCCH-COSY and HCCH-TOCSY spectra and the known protein backbone assignments. These assignments open the way to detailed studies of the sidechain structure and dynamics at the active site, in the hydrophobic core and on the surface of the protein.

PMID: 1901804 [PubMed - indexed for MEDLINE]



Source: PubMed