Multi-resonance NMR experiments are powerful analytical and structural tools. Their conceptualization assumes that RF fields may be combined independently to manipulate spin interactions. However, practical implementation can compromise performance. One limitation is the generation of combination bands when two or more RF fields are applied simultaneously within the NMR probe. The combination bands can lead to significant interference with the detection circuitry. A facile approach to combined multi-band decoupling can resolve these problems and increase sensitivity two-fold (or more), by time sharing the application of the individual frequencies rather than time sharing decoupling and data acquisition.
[NMR paper] Long-Observation-Window Band-Selective Homonuclear Decoupling: Increased Sensitivity and Resolution in Solid-State NMR Spectroscopy of Proteins
Long-Observation-Window Band-Selective Homonuclear Decoupling: Increased Sensitivity and Resolution in Solid-State NMR Spectroscopy of Proteins
Publication date: Available online 13 September 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Jochem O. Struppe , Chen Yang , Yachong Wang , Roy V. Hernandez , Lisa M. Shamansky , Leonard J. Mueller</br>
Sensitivity and resolution are the two fundamental obstacles to extending solid-state nuclear magnetic resonance to even larger protein systems. Here, a novel long-observation-window band-selective...
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09-13-2013 12:05 PM
[NMR paper] Refocused continuous-wave decoupling: a new approach to heteronuclear dipolar decoupling in solid-state NMR spectroscopy.
Refocused continuous-wave decoupling: a new approach to heteronuclear dipolar decoupling in solid-state NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--link.aip.org-jhtml-linkto.gif Related Articles Refocused continuous-wave decoupling: a new approach to heteronuclear dipolar decoupling in solid-state NMR spectroscopy.
J Chem Phys. 2012 Dec 7;137(21):214202
Authors: Vinther JM, Nielsen AB, Bjerring M, van Eck ER, Kentgens AP, Khaneja N, Nielsen NC
Abstract
A novel strategy for heteronuclear dipolar...
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06-01-2013 02:03 PM
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 22 September 2011</br>
Paul R.*Murray, David*Collison, Simon*Daff, Nicola*Austin, Ruth*Edge, ...</br>
A simple design for anin situ, three-electrode spectroelectrochemical cell is reported that can be used in commercial Q- and W-band (ca. 34 and 94 GHz, respectively) electron paramagnetic resonance (EPR) spectrometers, using standard sample tubing (1.0 and 0.5 mm inner diameter, respectively) and within variable temperature cryostat systems....
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09-24-2011 06:04 AM
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.
J Magn Reson. 2010 Dec 31;
Authors: Comellas G, Lopez JJ, Nieuwkoop AJ, Lemkau LR, Rienstra CM
We describe a simple yet highly effective optimization strategy for SPINAL-64 (1)H decoupling conditions for magic-angle spinning solid-state NMR. With...
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02-08-2011 06:28 PM
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR
Publication year: 2010
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 31 December 2010</br>
Gemma, Comellas , Jakob J., Lopez , Andrew J., Nieuwkoop , Luisel R., Lemkau , Chad M., Rienstra</br>
We describe a simple yet highly effective optimization strategy for SPINAL-64 1H decoupling conditions for magic-angle spinning solid-state NMR. With adjustment of the phase angles in a coupled manner,...
Combined multiā??band decoupling in biomolecular NMR spectroscopy
Linear Mode
