Combined frequency- and time-domain NMR spectroscopy. Application to fast protein res
 

Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment.

Related Articles Combined frequency- and time-domain NMR spectroscopy. Application to fast protein resonance assignment.

J Biomol NMR. 2004 May;29(1):57-64

Authors: Brutscher B

A simple and general method is presented to simplify multi-dimensional NMR spectra of isotope-labeled bio-molecules. The approach is based on band-selective Hadamard-type frequency encoding, which disperses the correlation peaks into different sub-spectra. This makes it possible to apply low-dimensionality-based NMR techniques to larger molecular systems. Here we demonstrate the use of band-selective Hadamard frequency labeling for fast protein resonance assignment, based on our recently proposed suite of 2D experiments (Bersch et al., 2003).

PMID: 15017139 [PubMed - indexed for MEDLINE]



Source: PubMed