A combinatorial selective labeling method for the assignment of backbone amide NMR re
 

A combinatorial selective labeling method for the assignment of backbone amide NMR resonances.

Related Articles A combinatorial selective labeling method for the assignment of backbone amide NMR resonances.

J Am Chem Soc. 2004 Apr 28;126(16):5020-1

Authors: Parker MJ, Aulton-Jones M, Hounslow AM, Craven CJ

A combinatorial selective labeling (CSL) method is presented for the assignment of backbone amide NMR resonances, which has a particular application in the identification of protein-ligand interaction sites. The method builds on the dual amino acid selective labeling technique. In the CSL method a number of different samples are produced, each with a different pattern of labeled amino acids. By analyzing peak intensities in HSQC and 2D HNCO spectra of these samples, a large number of combinations of amino acid pairs can be simultaneously assigned. We demonstrate the method on the 27 kDa protein GFP. The samples can be produced rapidly and cost-effectively in a commercially available in vitro translation system. The method greatly simplifies the process of backbone assignment and would be very straightforward to automate.

PMID: 15099056 [PubMed - indexed for MEDLINE]



Source: PubMed