Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.
 

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy.

Methods Mol Biol. 2018;1764:73-85

Authors: Ortega-Roldan JL, Blackledge M, Jensen MR

Abstract
In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis.


PMID: 29605909 [PubMed - in process]



More...