Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.
 

Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.

Characterizing protein-glycosaminoglycan interactions using solution NMR spectroscopy.

Methods Mol Biol. 2015;1229:325-33

Authors: Joseph PR, Poluri KM, Sepuru KM, Rajarathnam K

Abstract
Solution nuclear magnetic resonance (NMR) spectroscopy and, in particular, chemical shift perturbation (CSP) titration experiments are ideally suited for characterizing the binding interface of macromolecular complexes. (1)H-(15) N-HSQC-based CSP studies have become the method of choice due to their simplicity, short time requirements, and not requiring high-level NMR expertise. Nevertheless, CSP studies for characterizing protein-glycosaminoglycan (GAG) interactions have been challenging due to binding-induced aggregation/precipitation and/or poor quality data. In this chapter, we discuss how optimizing experimental variables such as protein concentration, GAG size, and sensitivity of NMR instrumentation can overcome these roadblocks to obtain meaningful structural insights into protein-GAG interactions.


PMID: 25325963 [PubMed - in process]



More...