Characterization of Ligand Binding by Saturation Transfer Difference NMR Spectroscopy.
 

Characterization of Ligand Binding by Saturation Transfer Difference NMR Spectroscopy.

Related Articles Characterization of Ligand Binding by Saturation Transfer Difference NMR Spectroscopy.

Angew Chem Int Ed Engl. 1999 Jun 14;38(12):1784-1788

Authors: Mayer M, Meyer B

Abstract
Fast identification of binding activity directly from mixtures of potential ligands is possible with the NMR method described, which is based on saturation transfer to molecules in direct contact to a protein. In addition, the ligand's binding epitope is easily identified. High sensitivity and ease of use are the principal advantages of this method. The picture shows the normal 1D NMR spectrum of a mixture and the spectrum obtained by applying the STD method, which exclusively shows signals from molecules with binding affinity.


PMID: 29711196 [PubMed]



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