In-cell protein NMR and protein leakage.
 

In-cell protein NMR and protein leakage.

In-cell protein NMR and protein leakage.

Proteins. 2010 Oct 11;

Authors: Barnes CO, Pielak GJ

In-cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in-cell NMR experiments and show that leakage is likely when the protein being studied exceeds ~20% of the total cellular protein. Proteins 2010. © 2010 Wiley-Liss, Inc.

PMID: 21120863 [PubMed - as supplied by publisher]



Source: PubMed