Calcium binding environments probed by (43)Ca NMR spectroscopy.

		BioNMR > Educational resources > Journal club
Calcium binding environments probed by (43)Ca NMR spectroscopy.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-16-2010, 09:21 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,175

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Calcium binding environments probed by (43)Ca NMR spectroscopy.

Calcium binding environments probed by (43)Ca NMR spectroscopy.

Calcium binding environments probed by (43)Ca NMR spectroscopy.

Dalton Trans. 2010 Oct 7;39(37):8593-602

Authors: Bryce DL

Calcium is an important component of materials, metalloproteins, minerals, glasses, and small inorganic and organic complexes. However, NMR spectroscopy of the quadrupolar (43)Ca nuclide remains difficult primarily due to its low natural abundance and low resonance frequency. In this Perspective, experimental challenges and recent successes in the field are highlighted, with a focus on solid-state (43)Ca NMR spectroscopy. Solution (43)Ca NMR studies of calcium-binding biomolecules are also presented. The structural insights afforded from quadrupolar and chemical shift parameters are examined. For example: isotropic chemical shifts have been shown to correlate with the mean Ca-O distance and also with calcium coordination number; quadrupolar coupling constants and chemical shift tensor spans have been shown to be useful probes of polymorphism; and, distance measurements involving (43)Ca have been recently demonstrated. Lastly, challenges and opportunities for the future are considered.

PMID: 20574585 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy. Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy. Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy. Methods Enzymol. 2010;478:305-22 Authors: Yamaguchi Y, Kato K Unique advantages offered by nuclear magnetic resonance (NMR) spectroscopy provide high-resolution information not only on structures but also on dynamics and interactions of glycoconjugates in solution. These benefits are further enhanced by applying stable-isotope-labeling techniques, which we...	nmrlearner	Journal club	0	12-31-2010 07:03 PM
[NMR paper] Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy. Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy. Related Articles Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy. Biochem Biophys Res Commun. 2005 Sep 23;335(2):361-6 Authors: Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC, Wilce JA Termination of DNA replication in Bacillus subtilis involves the polar arrest of replication forks by a specific complex formed between the dimeric 29 kDa replication terminator...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba H NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica. Related Articles NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica. Biochemistry. 2001 Dec 4;40(48):14392-403 Authors: Atreya HS, Sahu SC, Bhattacharya A, Chary KV, Govil G We present the three-dimensional (3D) solution structure of a calcium-binding protein from Entamoeba histolytica (EhCaBP), an etiologic agent of amoebiasis affecting millions worldwide. EhCaBP is a 14.7 kDa (134 residues) monomeric...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-int Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies. Related Articles Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies. J Mol Recognit. 2000 Mar-Apr;13(2):83-92 Authors: Hwang PM, Vogel HJ Calcium- and integrin-binding protein (CIB) binds to the...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis. Related Articles The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis. J Biol Chem. 1998 Sep 18;273(38):24701-7 Authors: Jansson M, Andersson G, Uhlén M, Nilsson B, Kördel J NMR spectroscopy studies and biosensor interaction analysis of native and site-directed mutants of insulin-like growth factor I (IGF-I) was...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy. Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy. Related Articles Supramolecular Interactions Probed by (13)C-(13)C Solid-State NMR Spectroscopy. J Am Chem Soc. 2010 Oct 8; Authors: Loquet A, Giller K, Becker S, Lange A We present a robust solid-state NMR approach for the accurate determination of molecular interfaces in insoluble and noncrystalline protein-protein complexes. The method relies on the measurement of intermolecular (13)C-(13)C distances in mixtures of glucose- and glucose-labeled proteins. We have...	nmrlearner	Journal club	0	10-12-2010 02:52 PM
Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy Supramolecular Interactions Probed by 13C-13C Solid-State NMR Spectroscopy Antoine Loquet, Karin Giller, Stefan Becker and Adam Lange http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107460j/aop/images/medium/ja-2010-07460j_0003.gif Journal of the American Chemical Society DOI: 10.1021/ja107460j http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/uejHS045F6M	nmrlearner	Journal club	0	10-09-2010 03:03 AM
[KPWU blog] [IDP] RTX calcium-binding motifs RTX calcium-binding motifs Title:RTX calcium-binding motifs are intrinsically disordered in the absence of calcium: Implication for protein secretion Authors: Alexandre Chenal, J. Iñaki Guijarro, Bertrand Raynal, Muriel Delepierre, and Daniel Ladant Journal: J. Biol. Chem, 10.1074/jbc.M807312200 (online publish first) Note: Not an NMR-based study.http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=194&subd=kpwu&ref=&feed=1 Go to KPWU blog to read complete post.	nmrlearner	News from NMR blogs	0	08-22-2010 01:32 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off