BSH-CP based 3D solid-state NMR experiments for protein resonance assignment
 

BSH-CP based 3D solid-state NMR experiments for protein resonance assignment

Abstract

We have recently presented band-selective homonuclear cross-polarization (BSH-CP) as an efficient method for COâ??CA transfer in deuterated as well as protonated solid proteins. Here we show how the BSH-CP COâ??CA transfer block can be incorporated in a set of three-dimensional (3D) solid-state NMR (ssNMR) pulse schemes tailored for resonance assignment of proteins at high static magnetic fields and moderate magic-angle spinning rates. Due to the achieved excellent transfer efficiency of 33Â*% for BSH-CP, a complete set of 3D spectra needed for unambiguous resonance assignment could be rapidly recorded within 1Â*week for the model protein ubiquitin. Thus we expect that BSH-CP could replace the typically used COâ??CA transfer schemes in well-established 3D ssNMR approaches for resonance assignment of solid biomolecules.



Source: Journal of Biomolecular NMR