Broadband 15Nâ??13C dipolar recoupling via symmetry-based RF pulse schemes at high MA
 

Abstract An approach for generating efficient
RNnnS, nk symmetry-based dual channel RF pulse schemes for γ-encoded broadband 15Nâ??13C dipolar recoupling at high magic angle spinning frequencies is presented. The method involves the numerical optimisation of the RF phase-modulation profile of the basic â??Râ?? element so as to obtain heteronuclear double quantum dipolar recoupling sequences with satisfactory magnetisation transfer characteristics. The basic â??Râ?? element was implemented as a sandwich of a small number of short pulses of equal duration with each pulse characterised by a RF phase and amplitude values. The performance characteristics of the sequences were evaluated via numerical simulations and 15Nâ??13C chemical shift correlation experiments. Employing such 13Câ??15N double-quantum recoupling sequences and the multiple receiver capabilities available in the current generation of NMR spectrometers, the possibility to simultaneously acquire 3D NCC and CNH chemical shift correlation spectra is also demonstrated.

• Content Type Journal Article
• DOI 10.1007/s10858-010-9406-z
• Authors
  • Christian Herbst, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Jirada Herbst, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Michela Carella, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Jörg Leppert, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Oliver Ohlenschläger, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Matthias Görlach, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany
  • Ramadurai Ramachandran, Fritz Lipmann Institute Research Group Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research 07745 Jena Germany

• • Journal Journal of Biomolecular NMR
  • Online ISSN 1573-5001
  • Print ISSN 0925-2738
  • Journal Volume Volume 47
  • Journal Issue Volume 47, Number 1

Source: Journal of Biomolecular NMR