Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and
 

Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values.

Related Articles Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values.

J Biomol NMR. 1999 Feb;13(2):187-91

Authors: Ottiger M, Bax A

It is demonstrated that mixtures of ditetradecyl-phosphatidylcholine or didodecyl-phoshatidylcholine and dihexyl-phosphatidylcholine in water from lyotropic liquid crystalline phases under similar conditions as previously reported for bicelles consisting of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC). The carboxy-ester bonds present in DMPC and DHPC are replaced by ether linkages in their alkyl analogs, which prevents acid- or base-catalyzed hydrolysis of these compounds. 15N-1H dipolar couplings measured for ubiquitin over the 2.3-10.4 pH range indicate that this protein retains a backbone conformation which is very similar to its structure at pH 6.5 over this entire range.

PMID: 10070759 [PubMed - indexed for MEDLINE]



Source: PubMed