Related ArticlesA ?-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL.
Proc Natl Acad Sci U S A. 2020 Aug 19;:
Authors: Schubeis T, Le Marchand T, Daday C, Kopec W, Tekwani Movellan K, Stanek J, Schwarzer TS, Castiglione K, de Groot BL, Pintacuda G, Andreas LB
Abstract
The protein AlkL is known to increase permeability of the outer membrane of bacteria for hydrophobic molecules, yet the mechanism of transport has not been determined. Differing crystal and NMR structures of homologous proteins resulted in a controversy regarding the degree of structure and the role of long extracellular loops. Here we solve this controversy by determining the de novo NMR structure in near-native lipid bilayers, and by accessing structural dynamics relevant to hydrophobic substrate permeation through molecular-dynamics simulations and by characteristic NMR relaxation parameters. Dynamic lateral exit sites large enough to accommodate substrates such as carvone or octane occur through restructuring of a barrel extension formed by the extracellular loops.
PMID: 32817429 [PubMed - as supplied by publisher]
[NMR paper] Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Related Articles Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Biomol NMR Assign. 2020 Jun 30;:
Authors: Schubeis T, Schwarzer TS, Le Marchand T, Stanek J, Movellan KT, Castiglione K, Pintacuda G, Andreas LB
Abstract
Most commonly small outer membrane proteins, possessing between 8 and 12 ?-strands, are not involved in transport but fulfill diverse functions...
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07-02-2020 11:18 AM
Effect of nitroxide spin probes on the transport properties of Nafion membranes #DNPNMR #ODNP
From The DNP-NMR Blog:
Effect of nitroxide spin probes on the transport properties of Nafion membranes #DNPNMR #ODNP
Überrück, Till, Oliver Neudert, Klaus-Dieter Kreuer, Bernhard Blümich, Josef Granwehr, Siegfried Stapf, and Songi Han. “Effect of Nitroxide Spin Probes on the Transport Properties of Nafion Membranes.” Physical Chemistry Chemical Physics 20, no. 41 (2018): 26660–74.
https://doi.org/10.1039/C8CP04607G.
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05-06-2019 04:47 PM
[NMR paper] Alamethicin Supramolecular Organization in Lipid Membranes from (19)F Solid-State NMR.
Alamethicin Supramolecular Organization in Lipid Membranes from (19)F Solid-State NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles Alamethicin Supramolecular Organization in Lipid Membranes from (19)F Solid-State NMR.
Biophys J. 2016 Dec 06;111(11):2450-2459
Authors: Salnikov ES, Raya J, De Zotti M, Zaitseva E, Peggion C, Ballano G, Toniolo C, Raap J, Bechinger B
Abstract
Alamethicins (ALMs) are antimicrobial peptides of fungal...
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07-05-2017 10:27 AM
Differential Coupling of Binding, ATP Hydrolysis, and Transport of Fluorescent Probes with P-Glycoprotein in Lipid Nanodiscs
Differential Coupling of Binding, ATP Hydrolysis, and Transport of Fluorescent Probes with P-Glycoprotein in Lipid Nanodiscs
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b01245/20170504/images/medium/bi-2016-01245n_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b01245
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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05-05-2017 09:06 AM
[NMR paper] Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR.
Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR.
Related Articles Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR.
J Biomol NMR. 2015 Jan 30;
Authors: Eddy MT, Su Y, Silvers R, Andreas L, Clark L, Wagner G, Pintacuda G, Emsley L, Griffin RG
Abstract
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01-31-2015 04:16 PM
Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR
Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR
Abstract
The human voltage dependent anion channel 1 (VDAC) is a 32Â*kDa β-barrel integral membrane protein that controls the transport of ions across the outer mitochondrial membrane. Despite the determination of VDAC solution and diffraction structures, a structural basis for the mechanism of its function is not yet fully understood. Biophysical studies suggest VDAC requires a lipid bilayer to achieve full function, motivating the need...
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01-30-2015 12:15 PM
[NMR paper] The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy.
The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy.
Chemistry. 2014 Mar 13;
Authors: Schmidt P, Thomas L, Müller P, Scheidt HA, Huster D
Abstract
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03-14-2014 07:34 PM
[NMR paper] Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes st
Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Related Articles Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Chem Phys Lipids. 2004 Nov;132(1):65-77
Authors: Grage SL, Afonin S, Grüne M, Ulrich AS
The interaction of the fusogenic polypeptide segment "B18" from the fertilization protein binding with lipid membranes was investigated by solid state 2H and 31P NMR, and by differential scanning calorimetry. B18 is known...
A ?-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL.
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