NMR paper Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids

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[NMR paper] Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids

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09-22-2016, 06:26 AM

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Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids

Band-selective heteronuclear dipolar recoupling with dual back-to-back pulses in rotating solids

Publication date: Available online 6 September 2016
Source:Journal of Magnetic Resonance

Author(s): Zhengfeng Zhang, Yanke Chen, Jun Yang

We propose a robust band-selective heteronuclear 15N-13C recoupling method using dual back-to-back (BABA) pulses (DBP). It contains four 90° pulses in each rotor period and corresponding phase cycling on each channel (13C and 15N). DBP aims at rapid band-selective heteronuclear magnetization transfer between 15N and 13C?/13C’, whose efficiency is close to that of the well-known SPECIFIC CP in membrane proteins with relatively short relaxation time in rotating frame (T 1?). Compared to SPECIFIC CP, DBP is very simple to set up and highly robust to RF variations. Thus, it can reduce the efforts in experimental optimization, especially for low-sensitive samples, and is very suitable for long-time or quantitative experiments. The efficacy of DBP is demonstrated by the E. coli diacylglycerol kinase (DAGK) proteoliposome. We anticipate that DBP would be useful for (segments of) membrane proteins that undergo the ?s-ms timescale motions in magic-angle spinning (MAS) solid-state NMR.
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