Mutational Analysis of the Binding-Induced FoldingReaction of the Mixed-Lineage Leukemia Protein to the KIX Domain

		BioNMR > Educational resources > Journal club
Mutational Analysis of the Binding-Induced FoldingReaction of the Mixed-Lineage Leukemia Protein to the KIX Domain

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-07-2016, 04:21 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,184

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Mutational Analysis of the Binding-Induced FoldingReaction of the Mixed-Lineage Leukemia Protein to the KIX Domain

Mutational Analysis of the Binding-Induced FoldingReaction of the Mixed-Lineage Leukemia Protein to the KIX Domain

Biochemistry
DOI: 10.1021/acs.biochem.6b00505

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
The RING domain of human promyelocytic leukemia protein (PML) The RING domain of human promyelocytic leukemia protein (PML) Source: Journal of Biomolecular NMR	nmrlearner	Journal club	0	01-28-2015 05:28 PM
Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain March 2012 Publication year: 2012 Source:Biochimie, Volume 94, Issue 3</br> </br> The insulin-like growth factor binding proteins are a family of six proteins (IGFBP-1 to -6) that bind insulin-like growth factors-I and -II (IGF-I/II) with high affinity. In addition to regulating IGF actions, IGFBPs have IGF-independent functions. IGFBP-2, the largest member of this family, is over-expressed in many cancers and has been proposed as a possible target for the...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Biochimie. 2011 Sep 22; Authors: Galea CA, Mobli M, McNeil KA, Mulhern TD, Wallace JC, King GF, Forbes BE, Norton RS Abstract The insulin-like growth factor binding proteins are a family of six proteins (IGFBP-1 to 6) that bind insulin-like growth factors-I and -II (IGF-I/II) with high affinity. In addition...	nmrlearner	Journal club	0	09-30-2011 06:00 AM
Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Insulin-like growth factor binding protein-2: NMR analysis and structural characterization of the N-terminal domain. Biochimie. 2011 Sep 22; Authors: Galea CA, Mobli M, McNeil KA, Mulhern TD, Wallace JC, King GF, Forbes BE, Norton RS Abstract The insulin-like growth factor binding proteins are a family of six proteins (IGFBP-1 to 6) that bind insulin-like growth factors-I and -II (IGF-I/II) with high affinity. In...	nmrlearner	Journal club	0	09-30-2011 05:59 AM
NMR analysis reveals 17?-estradiol induced conformational change in ER? ligand binding domain expressed in E. coli. NMR analysis reveals 17?-estradiol induced conformational change in ER? ligand binding domain expressed in E. coli. NMR analysis reveals 17?-estradiol induced conformational change in ER? ligand binding domain expressed in E. coli. Mol Biol Rep. 2010 Dec 12; Authors: Paramanik V, Thakur MK Nuclear magnetic resonance (NMR) spectroscopy is a useful biophysical technique to study the ligand-protein interaction. In this report, we have used bacterially produced ER? and its domains for studying the functional analysis of ligand-protein interaction....	nmrlearner	Journal club	0	12-15-2010 12:03 PM
[NMR paper] Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo- Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo-dGMP by the MutT pyrophosphohydrolase. Related Articles Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo-dGMP by the MutT pyrophosphohydrolase. Biochemistry. 2004 Mar 30;43(12):3404-14 Authors: Saraswat V, Azurmendi HF, Mildvan AS The solution structure of the ternary MutT enzyme-Mg(2+)-8-oxo-dGMP complex showed the proximity of Asn119 and Arg78 and the modified purine ring of 8-oxo-dGMP, suggesting specific roles for...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] 31P NMR analysis of the DNA conformation induced by protein binding SRY/DNA complexes 31P NMR analysis of the DNA conformation induced by protein binding SRY/DNA complexes. Related Articles 31P NMR analysis of the DNA conformation induced by protein binding SRY/DNA complexes. Eur J Biochem. 2000 Feb;267(4):1223-9 Authors: Castagné C, Murphy EC, Gronenborn AM, Delepierre M Complexes of the HMG box protein SRY with two duplexes of 8 and 14 base pairs have been studied by 31P NMR and complete assignment of all phosphorus signals of the bound DNA duplexes are presented. While for the free DNA, all 31P signals display limited...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] Mutational analysis and NMR spectroscopy of quail cysteine and glycine-rich protein C Mutational analysis and NMR spectroscopy of quail cysteine and glycine-rich protein CRP2 reveal an intrinsic segmental flexibility of LIM domains. Related Articles Mutational analysis and NMR spectroscopy of quail cysteine and glycine-rich protein CRP2 reveal an intrinsic segmental flexibility of LIM domains. J Mol Biol. 1999 Oct 1;292(4):893-908 Authors: Kloiber K, Weiskirchen R, Kräutler B, Bister K, Konrat R The LIM domain is a conserved cysteine and histidine-containing structural module of two tandemly arranged zinc fingers. It has been...	nmrlearner	Journal club	0	11-18-2010 08:31 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off