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Default Backbone (1)H, (15)N, (13)C and side chain (13)C? NMR chemical shift assignment of murine interleukin-10.

Backbone (1)H, (15)N, (13)C and side chain (13)C? NMR chemical shift assignment of murine interleukin-10.

Backbone (1)H, (15)N, (13)C and side chain (13)C? NMR chemical shift assignment of murine interleukin-10.

Biomol NMR Assign. 2013 Aug 25;

Authors: Künze G, Theisgen S, Huster D

Abstract
Almost complete assignment of backbone (1)H, (13)C, (15)N and side chain (13)C? resonances for the immune-regulatory cytokine IL-10 is reported. The protein was overexpressed in Escherichia coli and was refolded from inclusion bodies. The point mutation C149Y was introduced to suppress incorrect disulfide bond formation and to improve protein refolding.


PMID: 23982919 [PubMed - as supplied by publisher]



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