[NMR paper] Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Related Articles Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Annu Rev Biochem. 2014 Jun 2;83:291-315
Authors: Rosenzweig R, Kay LE
Abstract
Large macromolecular assemblies, so-called molecular machines, are critical to ensuring proper cellular function. Understanding how proper function is achieved at the atomic level is crucial to advancing multiple avenues of biomedical research. Biophysical studies often include X-ray diffraction and...
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06-07-2014 07:12 PM
Journal Highlight: Automatic assignment of 1H-NMR spectra of small molecules
Journal Highlight: Automatic assignment of 1H-NMR spectra of small molecules
http://www.spectroscopynow.com/common/images/thumbnails/1426b18dc06.jpgA novel data-evaluation procedure for the automatic atom to peak or multiplet assignment of 1H-NMR spectra of small molecules has been developed using a fast and robust expert system.
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11-18-2013 10:42 PM
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
Abstract While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decreasing extent of ordered structure, this task often becomes both difficult and time-consuming, and the recording of high-dimensional data with high-resolution may be essential. Random sampling of the evolution time space,...
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07-20-2012 11:13 PM
Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy
Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy
Abstract Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignment of methyl groups which still presents a very challenging and time-consuming process. Here we demonstrate that, in combination with a known 3D structure, paramagnetic...
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09-26-2011 06:42 AM
Site-Directed Methyl Group Labeling as an NMR Probe of Structure and Dynamics in Supramolecular Protein Systems: Applications to the Proteasome and to the ClpP Protease
Site-Directed Methyl Group Labeling as an NMR Probe of Structure and Dynamics in Supramolecular Protein Systems: Applications to the Proteasome and to the ClpP Protease
Tomasz L. Religa, Amy M. Ruschak, Rina Rosenzweig and Lewis E. Kay
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja202259a/aop/images/medium/ja-2011-02259a_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja202259a
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/rQfCMlQFoW8
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05-20-2011 09:17 PM
[NMR paper] A random graph approach to NMR sequential assignment.
A random graph approach to NMR sequential assignment.
Related Articles A random graph approach to NMR sequential assignment.
J Comput Biol. 2005 Jul-Aug;12(6):569-83
Authors: Bailey-Kellogg C, Chainraj S, Pandurangan G
Nuclear magnetic resonance (NMR) spectroscopy allows scientists to study protein structure, dynamics and interactions in solution. A necessary first step for such applications is determining the resonance assignment, mapping spectral data to atoms and residues in the primary sequence. Automated resonance assignment algorithms...
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12-01-2010 06:56 PM
[NMR paper] Use of graph theory for secondary structure recognition and sequential assignment in
Use of graph theory for secondary structure recognition and sequential assignment in heteronuclear (13C, 15N) NMR spectra: application to HU protein from Bacillus stearothermophilus.
Related Articles Use of graph theory for secondary structure recognition and sequential assignment in heteronuclear (13C, 15N) NMR spectra: application to HU protein from Bacillus stearothermophilus.
Biopolymers. 1996 Nov;39(5):691-707
Authors: van Geerestein-Ujah EC, Mariani M, Vis H, Boelens R, Kaptein R
A computer-assisted procedure, based upon a branch of...
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08-22-2010 02:20 PM
[NMR paper] Graph-theoretical assignment of secondary structure in multidimensional protein NMR s
Graph-theoretical assignment of secondary structure in multidimensional protein NMR spectra: application to the lac repressor headpiece.
Related Articles Graph-theoretical assignment of secondary structure in multidimensional protein NMR spectra: application to the lac repressor headpiece.
J Biomol NMR. 1995 Jul;6(1):67-78
Authors: van Geerestein-Ujah EC, Slijper M, Boelens R, Kaptein R
A novel procedure is presented for the automatic identification of secondary structures in proteins from their corresponding NOE data. The method uses a branch...
Automatic Assignment of Methyl-NMR Spectra of Supramolecular Machines Using Graph Theory
Linear Mode
