Atomic view of cosolute-induced protein denaturation probed by NMR solvent paramagnetic relaxation enhancement [Chemistry]
 

Atomic view of cosolute-induced protein denaturation probed by NMR solvent paramagnetic relaxation enhancement [Chemistry]

Yusuke Okuno, Janghyun Yoo, Charles D. Schwieters, Robert B. Best, Hoi Sung Chung, G. Marius Clore...
Date: 2021-08-17

The cosolvent effect arises from the interaction of cosolute molecules with a protein and alters the equilibrium between native and unfolded states. Denaturants shift the equilibrium toward the latter, while osmolytes stabilize the former. The molecular mechanism whereby cosolutes perturb protein stability is still the subject of considerable debate. Probing... Read More


PNAS:
Number: 34
Volume: 118