[NMR paper] Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR
Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR
[NMR paper] Conformational Changes in Ff Phage Protein gVp upon Complexation with Its Viral Single-Stranded DNA Revealed Using Magic-Angle Spinning Solid-State NMR
Conformational Changes in Ff Phage Protein gVp upon Complexation with Its Viral Single-Stranded DNA Revealed Using Magic-Angle Spinning Solid-State NMR
Gene V protein (gVp) of the bacteriophages of the Ff family is a non-specific single-stranded DNA (ssDNA) binding protein. gVp binds to viral DNA during phage replication inside host Escherichia coli cells, thereby blocking further replication and signaling the assembly of new phage particles. gVp is a dimer in solution and in crystal form. A structural model of the complex between gVp and ssDNA was obtained via docking the free gVp to...
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06-24-2022 11:45 PM
[NMR paper] Deuteration for High-Resolution Detection of Protons in Protein Magic Angle Spinning (MAS) Solid-State NMR
Deuteration for High-Resolution Detection of Protons in Protein Magic Angle Spinning (MAS) Solid-State NMR
Proton detection developed in the last 20 years as the method of choice to study biomolecules in the solid state. In perdeuterated proteins, proton dipolar interactions are strongly attenuated, which allows yielding of high-resolution proton spectra. Perdeuteration and backsubstitution of exchangeable protons is essential if samples are rotated with MAS rotation frequencies below 60 kHz. Protonated samples can be investigated directly without spin dilution using proton detection...
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12-07-2021 02:05 PM
[NMR paper] Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR.
Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif Related Articles Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR.
Nat Struct Mol Biol. 2020 Sep;27(9):863-869
Authors: Lu M, Russell RW, Bryer AJ, Quinn CM, Hou G, Zhang H, Schwieters CD, Perilla JR, Gronenborn AM, Polenova T
Abstract
HIV-1 capsid plays multiple key roles in viral replication, and inhibition of capsid...
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09-13-2020 09:18 AM
[NMR paper] Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination.
Methods Mol Biol. 2018;1688:67-97
Authors: Morag O, Sgourakis NG, Abramov G, Goldbourt A
Abstract
Filamentous bacteriophages are...
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11-21-2017 10:10 PM
[NMR paper] Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14611-6
Authors: Yan S, Guo C, Hou G, Zhang H, Lu X, Williams JC, Polenova T
Abstract
Microtubules and their associated proteins perform a broad array of essential physiological functions, including...
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11-26-2015 12:22 PM
[NMR paper] Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
NMR Biomed. 2015 Mar 12;
Authors: Tufts L, Shet Vishnudas K, Fu E, Kurhanewicz J, Ries M, Alliston T, Li X
Abstract
Osteoarthritis (OA) is a common multifactorial and heterogeneous degenerative joint disease, and biochemical changes in cartilage matrix occur during the early stages of OA before...
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03-13-2015 12:31 PM
[NMR paper] Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
Pf1 bacteriophage hydration by magic angle spinning solid-state NMR.
J Chem Phys. 2014 Dec 14;141(22):22D533
Authors: Sergeyev IV, Bahri S, Day LA, McDermott AE
Abstract
High resolution two- and three-dimensional heteronuclear correlation spectroscopy ((1)H-(13)C, (1)H-(15)N, and (1)H-(13)C-(13)C HETCOR) has provided a detailed characterization of the internal and external hydration water of the Pf1 virion. This long and slender virion (2000 nm × 7 nm) contains...
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12-17-2014 09:43 PM
[NMR paper] Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscop
Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy.
Related Articles Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy.
Nature. 2002 Nov 7;420(6911):98-102
Authors: Castellani F, van Rossum B, Diehl A, Schubert M, Rehbein K, Oschkinat H
The determination of a representative set of protein structures is a chief aim in structural genomics. Solid-state NMR may have a crucial role in structural investigations of those proteins that do not easily form crystals or are not...
Atomic-Resolution Structure of the Protein Encoded by Gene V of fd Bacteriophage in Complex with Viral ssDNA Determined by Magic-Angle Spinning Solid-State NMR
Linear Mode
