Related ArticlesAssessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by ¹H HR-MAS NMR Metabolomics.
Int J Mol Sci. 2016 Oct 31;17(11):
Authors: Tavel L, Fontana F, Garcia Manteiga JM, Mari S, Mariani E, Caneva E, Sitia R, Camnasio F, Marcatti M, Cenci S, Musco G
Abstract
Multiple myeloma (MM) is a malignancy of plasma cells characterized by multifocal osteolytic bone lesions. Macroscopic and genetic heterogeneity has been documented within MM lesions. Understanding the bases of such heterogeneity may unveil relevant features of MM pathobiology. To this aim, we deployed unbiased ¹H high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) metabolomics to analyze multiple biopsy specimens of osteolytic lesions from one case of pathological fracture caused by MM. Multivariate analyses on normalized metabolite peak integrals allowed clusterization of samples in accordance with a posteriori histological findings. We investigated the relationship between morphological and NMR features by merging morphological data and metabolite profiling into a single correlation matrix. Data-merging addressed tissue heterogeneity, and greatly facilitated the mapping of lesions and nearby healthy tissues. Our proof-of-principle study reveals integrated metabolomics and histomorphology as a promising approach for the targeted study of osteolytic lesions.
[NMR paper] NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions.
NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions.
NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions.
Biochemistry. 2016 Jun 20;
Authors: Zalesak J, Constant JF, Jourdan M
Abstract
Ionizing radiations, free radicals and reactive oxygen species lead to hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiations. They compromise the efficiency of the base excision repair pathway (BER) and as a consequence,...
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Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
ABSTRACT
By applying - and -glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray structure and 1.32 Å to the...
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02-02-2015 09:55 PM
[NMR paper] Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy.
Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy.
Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy.
J Magn Reson. 2014 Jun 28;245C:98-104
Authors: Das BB, Opella SJ
Abstract
Multiple acquisition spectroscopy (MACSY) experiments that enable multiple free induction decays to be recorded during individual experiments are demonstrated. In...
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07-16-2014 10:46 AM
[NMR paper] Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
J Phys Chem B. 2014 Mar 14;
Authors: Calligari PA, Abergel D
Abstract
Fluctuations of NMR resonance frequency shifts and their relation with protein exchanging conformations are usually...
NMR solution structures of clustered abasic site lesions in DNA: structural differenc
NMR solution structures of clustered abasic site lesions in DNA: structural differences between 3'-staggered (-3) and 5'-staggered (+3) bistranded lesions.
Related Articles NMR solution structures of clustered abasic site lesions in DNA: structural differences between 3'-staggered (-3) and 5'-staggered (+3) bistranded lesions.
Biochemistry. 2010 Oct 19;49(41):8978-87
Authors: Hazel RD, de los Santos C
Ionizing radiation produces a distinctive pattern of bistranded clustered lesions in DNA. A relatively low number of clustered lesions may be lethal...
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11-04-2010 10:07 AM
NMR Solution Structures of Clustered Abasic Site Lesions in DNA: Structural Differenc
NMR Solution Structures of Clustered Abasic Site Lesions in DNA: Structural Differences between 3?-Staggered (-3) and 5?-Staggered (+3) Bistranded Lesions
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi101021e/aop/images/medium/bi-2010-01021e_0010.gif
Biochemistry
DOI: 10.1021/bi101021e
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by ¹H HR-MAS NMR Metabolomics.
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