BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 04-04-2017, 10:41 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 18,077
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by H HR-MAS NMR Metabolomics.

Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by H HR-MAS NMR Metabolomics.

Related Articles Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by H HR-MAS NMR Metabolomics.

Int J Mol Sci. 2016 Oct 31;17(11):

Authors: Tavel L, Fontana F, Garcia Manteiga JM, Mari S, Mariani E, Caneva E, Sitia R, Camnasio F, Marcatti M, Cenci S, Musco G

Abstract
Multiple myeloma (MM) is a malignancy of plasma cells characterized by multifocal osteolytic bone lesions. Macroscopic and genetic heterogeneity has been documented within MM lesions. Understanding the bases of such heterogeneity may unveil relevant features of MM pathobiology. To this aim, we deployed unbiased H high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) metabolomics to analyze multiple biopsy specimens of osteolytic lesions from one case of pathological fracture caused by MM. Multivariate analyses on normalized metabolite peak integrals allowed clusterization of samples in accordance with a posteriori histological findings. We investigated the relationship between morphological and NMR features by merging morphological data and metabolite profiling into a single correlation matrix. Data-merging addressed tissue heterogeneity, and greatly facilitated the mapping of lesions and nearby healthy tissues. Our proof-of-principle study reveals integrated metabolomics and histomorphology as a promising approach for the targeted study of osteolytic lesions.


PMID: 27809247 [PubMed - indexed for MEDLINE]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions.
NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions. NMR solution structure of DNA featuring clustered 2'-deoxyribonolactone and 8-oxoguanine lesions. Biochemistry. 2016 Jun 20; Authors: Zalesak J, Constant JF, Jourdan M Abstract Ionizing radiations, free radicals and reactive oxygen species lead to hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiations. They compromise the efficiency of the base excision repair pathway (BER) and as a consequence,...
nmrlearner Journal club 0 06-21-2016 01:30 PM
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR
Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR ABSTRACT By applying - and -glucose labeling schemes to the folded globular protein ubiquitin, a strong reduction of spectral crowding and increase in resolution in solid-state NMR (ssNMR) spectra could be achieved. This allowed spectral resonance assignment in a straightforward manner and the collection of a wealth of long-range distance information. A high precision solid-state NMR structure of microcrystalline ubiquitin was calculated with a backbone rmsd of 1.57 to the X-ray structure and 1.32 to the...
nmrlearner Journal club 0 02-02-2015 09:55 PM
[NMR paper] Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy.
Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy. Multiple acquisition/multiple observation separated local field/chemical shift correlation solid-state magic angle spinning NMR spectroscopy. J Magn Reson. 2014 Jun 28;245C:98-104 Authors: Das BB, Opella SJ Abstract Multiple acquisition spectroscopy (MACSY) experiments that enable multiple free induction decays to be recorded during individual experiments are demonstrated. In...
nmrlearner Journal club 0 07-16-2014 10:46 AM
[NMR paper] Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts. J Phys Chem B. 2014 Mar 14; Authors: Calligari PA, Abergel D Abstract Fluctuations of NMR resonance frequency shifts and their relation with protein exchanging conformations are usually...
nmrlearner Journal club 0 03-19-2014 10:43 PM
[Stan NMR blog] Metabolomics of Intact Tissues: Discrimination Between Osteolytic Lesion Regions ...
Metabolomics of Intact Tissues: Discrimination Between Osteolytic Lesion Regions ... A nice and novel application of NMR in clinical medicine (2012 poster). Source: Stan blog library
nmrlearner News from NMR blogs 0 01-21-2014 11:10 PM
[Stan NMR blog] Metabolomics of Intact Tissues: ... Osteolytic Lesions ...
Metabolomics of Intact Tissues: ... Osteolytic Lesions ... A nice and novel application of NMR in clinical medicine (2012 poster). Source: Stan blog library
nmrlearner News from NMR blogs 0 01-19-2014 02:38 AM
NMR solution structures of clustered abasic site lesions in DNA: structural differenc
NMR solution structures of clustered abasic site lesions in DNA: structural differences between 3'-staggered (-3) and 5'-staggered (+3) bistranded lesions. Related Articles NMR solution structures of clustered abasic site lesions in DNA: structural differences between 3'-staggered (-3) and 5'-staggered (+3) bistranded lesions. Biochemistry. 2010 Oct 19;49(41):8978-87 Authors: Hazel RD, de los Santos C Ionizing radiation produces a distinctive pattern of bistranded clustered lesions in DNA. A relatively low number of clustered lesions may be lethal...
nmrlearner Journal club 0 11-04-2010 10:07 AM
NMR Solution Structures of Clustered Abasic Site Lesions in DNA: Structural Differenc
NMR Solution Structures of Clustered Abasic Site Lesions in DNA: Structural Differences between 3?-Staggered (-3) and 5?-Staggered (+3) Bistranded Lesions http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi101021e/aop/images/medium/bi-2010-01021e_0010.gif Biochemistry DOI: 10.1021/bi101021e http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/GNgtLWuPbdM More...
nmrlearner Journal club 0 09-21-2010 06:43 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 03:09 PM.


Map