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NMR processing:
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Side-chains:
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NOEs:
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Ab initio:
GeNMR
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Refinement:
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Torsion angles from chemical shifts:
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Secondary structure from chemical shifts:
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Flexibility from chemical shifts:
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Chemical shifts re-referencing:
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From structure:
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From sequence:
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Disordered proteins:
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Format conversion & validation:
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From NMR-STAR 3.1
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Protein disorder:
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Protein solubility:
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Default Application of difference NOE-pumping NMR technique and cold-spray ionization mass sp

Application of difference NOE-pumping NMR technique and cold-spray ionization mass spectrometry to identify a ligand binding with a protein receptor.

Related Articles Application of difference NOE-pumping NMR technique and cold-spray ionization mass spectrometry to identify a ligand binding with a protein receptor.

Anal Sci. 2004 Oct;20(10):1467-70

Authors: Seki H, Sei Y, Shikii K, Shimotakahara S, Utsumi H, Yamaguchi K, Tashiro M

A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.

PMID: 15524205 [PubMed - indexed for MEDLINE]



Source: PubMed
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