Metallothioneins constitute a class of ubiquitously occurring low molecular mass proteins (6-7 kDa) possessing two cysteine thiolate-based metal clusters usually formed by the preferential binding of d10 metal ions such as ZnII and CdII. The three-dimensional solution structure of mammalian proteins has been determined by two-dimensional NMR spectroscopy of 113Cd7-metallothionein. The structure shows two protein domains encompassing the M3(CysS)9- and M4(CysS)11-cluster with each metal ion being tetrahedrally coordinated by thiolate ligands. The application of 113Cd NMR proved to be indispensable in the structural studies of metallothioneins. Thus, both homonuclear 113Cd decoupling studies and 113Cd-113Cd COSY of 113Cd7-metallothionein established the existence of two metal-thiolate clusters in this protein. The identification of sequence specific cysteine-cadmium coordinative bonds came from heteronuclear 113Cd-1H COSY experiments. Independently, the 113Cd NMR characterization of the intermediate metal-protein complexes, leading to the cluster structure in 113Cd7-metallothionein, revealed a stepwise cluster formation process with the Cd4(CysS)11-cluster being formed first. The recent demonstration of a Karplus-like dependence between the heteronuclear3 J(113Cd, 1H) coupling constants for the cysteine C beta protons and the H beta-C beta-S gamma -Cd dihedral angles should allow to derive the geometry of the CD-(S-Cys) centers in various metallothioneins and related metalloproteins. A possible application of 113Cd NMR to the study of metallothioneins in the environment is discussed.
[NMR paper] NMR determination of the global structure of the 113Cd derivative of desulforedoxin:
NMR determination of the global structure of the 113Cd derivative of desulforedoxin: investigation of the hydrogen bonding pattern at the metal center.
Related Articles NMR determination of the global structure of the 113Cd derivative of desulforedoxin: investigation of the hydrogen bonding pattern at the metal center.
Protein Sci. 1998 Apr;7(4):928-37
Authors: Goodfellow BJ, Rusnak F, Moura I, Domke T, Moura JJ
Desulforedoxin (Dx) is a simple homodimeric protein isolated from Desulfovibrio gigas (Dg) containing a distorted rubredoxin-like...
nmrlearner
Journal club
0
11-17-2010 11:06 PM
Application of LC-NMR and HR-NMR to the characterization of biphenyl impurities in th
Application of LC-NMR and HR-NMR to the characterization of biphenyl impurities in the synthetic route development for vestipitant, a novel NK1 antagonist.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Application of LC-NMR and HR-NMR to the characterization of biphenyl impurities in the synthetic route development for vestipitant, a novel NK1 antagonist.
J Pharm Biomed Anal. 2010 Nov 2;53(3):389-95
Authors: Provera S, Martini L, Guercio G, Turco L, Costa L, Marchioro C
...
nmrlearner
Journal club
0
09-19-2010 03:08 AM
[NMR paper] Copper- and silver-substituted yeast metallothioneins: sequential 1H NMR assignments
Copper- and silver-substituted yeast metallothioneins: sequential 1H NMR assignments reflecting conformational heterogeneity at the C terminus.
Related Articles Copper- and silver-substituted yeast metallothioneins: sequential 1H NMR assignments reflecting conformational heterogeneity at the C terminus.
Biochemistry. 1993 Jul 6;32(26):6773-87
Authors: Narula SS, Winge DR, Armitage IM
Complete 1H NMR sequential assignments have been made for copper(I)- and silver (I)-substituted metallothionein (MT) from Saccharomyces cerevisiae using standard...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] 113Cd-NMR investigation of a cadmium-substituted copper, zinc-containing superoxide d
113Cd-NMR investigation of a cadmium-substituted copper, zinc-containing superoxide dismutase from yeast.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 113Cd-NMR investigation of a cadmium-substituted copper, zinc-containing superoxide dismutase from yeast.
Eur J Biochem. 1991 Jun 15;198(3):607-11
Authors: Kofod P, Bauer R, Danielsen E, Larsen E, Bjerrum MJ
113Cd nuclear magnetic resonance spectroscopy has been used to...
Application of 113Cd NMR to metallothioneins.
Linear Mode
