Analyzing paramagnetic NMR data on target DNA search by proteins using a discrete-state kinetic model for translocation
 

Analyzing paramagnetic NMR data on target DNA search by proteins using a discrete-state kinetic model for translocation

Before reaching their targets, sequence-specific DNA-binding proteins nonspecifically bind to DNA through electrostatic interactions and stochastically change their locations on DNA. Investigations into the dynamics of DNA-scanning by proteins are nontrivial due to the simultaneous presence of multiple translocation mechanisms and many sites for the protein to nonspecifically bind to DNA. Nuclear magnetic resonance (NMR) spectroscopy can provide information about the target DNA search processes...

More...