Analysis of the Effects of ?-Tocopherol (?-TOH) on RAW264.7 and K562 Cells Based on 1H-NMR Metabonomics.
 

Analysis of the Effects of ?-Tocopherol (?-TOH) on RAW264.7 and K562 Cells Based on 1H-NMR Metabonomics.

Analysis of the Effects of ?-Tocopherol (?-TOH) on RAW264.7 and K562 Cells Based on 1H-NMR Metabonomics.

J Agric Food Chem. 2018 Jan 09;:

Authors: Lu Y, Li H, Geng Y

Abstract
?-Tocopherol (?-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of ?-TOH using the IC50 of ?-TOH on RAW264.7 (80 ?M) and K562 (110 ?M) cells. We compared the differential metabolites from the cell lines with and without ?-TOH treatment by 1H-NMR metabonomics analysis. It was found that ?-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with ?-TOH as differential metabolites were produced. The betaine level in RAW264.7 cells was reduced significantly while the L-lactic acid level in K562 cells was significantly enhanced. The metabolic changes might contribute to the switch of the respiration pattern from aerobic respiration to anaerobic respiration in K562 cell. These results are helpful in a further understanding of the sub-toxicity of ?-TOH.


PMID: 29313349 [PubMed - as supplied by publisher]



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