[NMR paper] Agronomic characterization and ?- and ?-ODAP determination through the adoption of new analytical strategies (HPLC-ELSD and NMR) of ten Sicilian accessions of grass pea.
Agronomic characterization and ?- and ?-ODAP determination through the adoption of new analytical strategies (HPLC-ELSD and NMR) of ten Sicilian accessions of grass pea.
Related ArticlesAgronomic characterization and ?- and ?-ODAP determination through the adoption of new analytical strategies (HPLC-ELSD and NMR) of ten Sicilian accessions of grass pea.
Abstract
Ten accessions of grass pea (Lathyrus sativus L.) from different Sicilian sites, cultivated in the same environmental conditions were analyzed for their morphological and productive parameters and for the content of two non-protein amino acids: ?- and ?-ODAP(?- and ?-N-oxalyl-L-?,?-diaminopropionic acid). The ?-isomer is the neurotoxin responsible for the neuron disease known as lathyrism. This analysis was carried out using two common analytical methodologies never applied in their determination, an HPLC separation with evaporative light scattering (ELS) as detector, and nuclear magnetic resonance (NMR). The content of the two isomers falls in the range reported for these compounds: 0.42 - 0.74 and 2.69 - 4.59 g/kg for ?- and ?-ODAP, respectively; and results with the two methods yield comparable results. High productivity and a high protein content were detected in three Sicilian accessions. Low ?-ODAP content was found to be linked to accessions with heavier seeds and those originating at lower altitudes.
PMID: 24559213 [PubMed - as supplied by publisher]
[NMR paper] Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
Related Articles Characterization of potential degradation products in a PEGylating reagent 20kDa monomethoxy polyethylene glycol propionaldehyde by RP-HPLC, APCI-MS and NMR.
J Pharm Biomed Anal. 2013 Nov 15;89C:221-226
Authors: Zhang H, Wilson J, Zhang J, Luo Y
Abstract
Ensuring quality of PEGylating reagents is essential for the successful development and manufacturing of PEGylated...
Industrial Postdoctoral Chemist( analytical, organic or polymer) | Avomeen Analytical Services
Industrial Postdoctoral Chemist( analytical, organic or polymer) | Avomeen Analytical Services
US - Ann Arbor Michigan, Ph.D. in Analytical, organic or polymer chemistry. Hands-on knowledge of following techniques Analytical instrumentation is preferred: FT-IR, NMR, GC, GC-MS, LC-MS, TGA, DSC, ICP, and SEM-EDS. Must be
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[NMR paper] Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Related Articles Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Magn Reson Chem. 2005 Sep;43(9):747-54
Authors: Hentschel P, Krucker M, Grynbaum MD, Putzbach K, Bischoff R, Albert K
The protein kinase ZAP-70 is involved in T-cell activation and interacts with...
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[NMR paper] NMR characterization and solution structure determination of the oxidized cytochrome
NMR characterization and solution structure determination of the oxidized cytochrome c7 from Desulfuromonas acetoxidans.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR characterization and solution structure determination of the oxidized cytochrome c7 from Desulfuromonas acetoxidans.
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14396-400
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Agronomic characterization and ?- and ?-ODAP determination through the adoption of new analytical strategies (HPLC-ELSD and NMR) of ten Sicilian accessions of grass pea.
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