Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
 

Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins

Detergent-soluble proteins (DSPs) are commonly dissolved in lipid buffers for NMR experiments, but the huge lipid proton signal prevents recording of high-quality spectra. The use of costly deuterated lipids is thus required to replace non-deuterated ones. With conventional methods, detergents like dodecylphosphocholine (DPC) cannot be fully exchanged due to their high binding affinity to hydrophobic proteins. We propose an original and simple protocol which combines the use of acetonitrile,...

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