3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy. Chem Commun (Camb). 2016 Jul 29; Authors: Pan BB, Yang F, Ye Y, Wu Q, Li C, Huber T, Su XC Abstract Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes. PMID: 27470136 [PubMed - as supplied by publisher] More... |
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