2'-(19)F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions
 

2'-(19)F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions

Protein-RNA interactions are central to numerous cellular processes. In this work, we present an easy and straightforward NMR-based approach to determine the RNA binding site of RNA binding proteins and to evaluate the binding of pairs of proteins to a single-stranded RNA (ssRNA) under physiological conditions, in this case in nuclear extracts. By incorporation of a ^(19)F atom on the ribose of different nucleotides along the ssRNA sequence, we show that, upon addition of an RNA binding protein,...

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